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16 March 2023 Polychromatic digital holographic microscopy for live-cell imaging (Conference Presentation)
Céline Larivière-Loiselle, Mohamed Haouat, Erik Bélanger, Pierre Marquet
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Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC123910E (2023) https://doi.org/10.1117/12.2647770
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We have developed a polychromatic digital holographic microscope (P-DHM) as a quasi-speckle-free quantitative-phase imaging, having preserved its nanometric sensitivity from interferometric detection, for live-cell imaging. With this approach, we can visualize tiny subcellular structures and monitor various fine cellular dynamic events including neuronal branching and connectivity rearrangement, lamellipodia movements and cellular trafficking. We are working to reach a higher frame-rate to image faster-acting cellular phenomena like neuronal excitation.
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Céline Larivière-Loiselle, Mohamed Haouat, Erik Bélanger, and Pierre Marquet "Polychromatic digital holographic microscopy for live-cell imaging (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC123910E (16 March 2023); https://doi.org/10.1117/12.2647770
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KEYWORDS
Digital holography
Holography
Microscopy
Cell mechanics
Holograms
Tunable lasers
Video
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