Advancing low flux in vivo two-photon voltage imaging (Conference Presentation)

Jerry Chen

doi:10.1117/12.2656201

16 March 2023 Advancing low flux in vivo two-photon voltage imaging (Conference Presentation)

Jerry Chen

Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC1239007 (2023) https://doi.org/10.1117/12.2656201
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Monitoring spiking activity across large neuronal populations at behaviorally relevant timescales is critical for understanding neural circuit function. Voltage imaging requires kilohertz sampling rates which reduce fluorescence detection to near shot noise levels. High-photon flux excitation can overcome photon-limited shot noise but photo-bleaching and photo-damage restrict the number and duration of simultaneously imaged neurons. We investigated an alternative approach aimed at low two-photon flux, voltage imaging below the shot noise limit with the goal of achieving simultaneous high-speed, deep-tissue imaging of more than one hundred densely labeled neurons over one hour in awake behaving mice.

Conference Presentation

Citation Download Citation

Jerry Chen "Advancing low flux in vivo two-photon voltage imaging (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC1239007 (16 March 2023); https://doi.org/10.1117/12.2656201

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