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15 March 2023 Micro-optical coherence tomography-confocal fluorescence microscopy (µOCT-FCM) for probing cystic fibrosis pathogenesis (Conference Presentation)
Linhui Yu, Courtney F. Petty, Hui Min Leung, Susan E. Birket, Steven M. Rowe, Guillermo J. Tearney
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Proceedings Volume PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; PC123830B (2023) https://doi.org/10.1117/12.2650168
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Cystic fibrosis is the most common fatal genetic disorder in the US. In airways with CF, bicarbonate concentration affects airway liquid pH, which then modulates mucogenesis. Causative relationships between these chemical parameters and the functional microanatomy during pathogenesis and disease progression are unclear due to the lack of quantitative tools suitable for in situ studies. We utilized a new µOCT-FCM system to measure the airway functional microanatomy parameters and subsequent pH in the mucus in situ in a non-contact manner. We demonstrated the quantification of pH of the ASL using SNARF-1 in a spatially resolved manner. The µOCT-FCM imaging allowed us to visualize pH in the mucus compartments of the airway and achieved colocalized μOCT and fluorescence imaging. In human bronchial epithelial cells, CF cell ASL pH values were lower than those in the non-CF cells as expected. Pilot results from ex-vivo swine trachea showed that tracheal mucus is heterogeneous.
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Linhui Yu, Courtney F. Petty, Hui Min Leung, Susan E. Birket, Steven M. Rowe, and Guillermo J. Tearney "Micro-optical coherence tomography-confocal fluorescence microscopy (µOCT-FCM) for probing cystic fibrosis pathogenesis (Conference Presentation)", Proc. SPIE PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, PC123830B (15 March 2023); https://doi.org/10.1117/12.2650168
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KEYWORDS
Luminescence
Cystic fibrosis
Pathogens
Microscopy
Liquids
Optical coherence tomography
Optical filters
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