Deep-tissue photoacoustic imaging and optogenetic manipulation of a near-infrared photoswitchable transgenic mouse model

Chenshuo Ma; Ludmila A. Kasatkina; Mikhail E. Matlashov; Tri Vu; Mucong Li; Andrii A. Kaberniuk; Junjie Yao; Vladislav V. Verkhusha

doi:10.1117/12.2648543

9 March 2023 Deep-tissue photoacoustic imaging and optogenetic manipulation of a near-infrared photoswitchable transgenic mouse model

Chenshuo Ma, Ludmila A. Kasatkina, Mikhail E. Matlashov, Tri Vu, Mucong Li, Andrii A. Kaberniuk, Junjie Yao, Vladislav V. Verkhusha

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Proceedings Volume PC12379, Photons Plus Ultrasound: Imaging and Sensing 2023; PC123791A (2023) https://doi.org/10.1117/12.2648543
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Our study exploits Rhodopseudomonas palustris BphP1 bacterial phytochrome to generate a near-infrared (NIR) loxP-BphP1 photoswitchable transgenic mouse model that enables deep-tissue optogenetics and photoacoustic tomography (PAT). BphP1 incorporates biliverdin and reversibly switches between the ground state and activated state, with distinct optical absorption spectra in the NIR window. We validated the optogenetic performance of the BphP1-encoded mouse model to trigger gene transcription, and demonstrated its superior capability of deep-tissue optogenetics. Then, taking advantage of BphP1's photoswitching properties, we can suppress the non-switching signals from background blood and improve the molecular detection sensitivity of PAT by three orders of magnitude.

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Chenshuo Ma, Ludmila A. Kasatkina, Mikhail E. Matlashov, Tri Vu, Mucong Li, Andrii A. Kaberniuk, Junjie Yao, and Vladislav V. Verkhusha "Deep-tissue photoacoustic imaging and optogenetic manipulation of a near-infrared photoswitchable transgenic mouse model", Proc. SPIE PC12379, Photons Plus Ultrasound: Imaging and Sensing 2023, PC123791A (9 March 2023); https://doi.org/10.1117/12.2648543

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KEYWORDS

Optogenetics

Mouse models

Near infrared

Photoacoustic imaging

Acquisition tracking and pointing

Absorption

In vivo imaging

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