In traditional Chinese medicine (TCM), it is widely accepted that acupoints on meridian not only represent the treatment points but also serve as diagnostic points. We exploited our existing technology of multi-channel deep tissue microcirculation measurement known as diffuse speckle contrast analysis (DSCA) to see 1) whether a pressure stimulus on specific acupoint causes microcirculation change in other acupoints on the same meridian, and 2) whether the change is position-dependent. The experimental setup has been validated by cuff occlusion protocol, and then a pressure was applied on LI4 of human subjects several times with a regular time interval. We monitored, on acupoints of LI1, LI5, LI10, and ST25, not only the average blood flow index (BFI) but also the change of complexity in low frequency oscillation (LFO) signal which is known to be related to local tissue viability.
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