Dynamic optical coherence tomography for tumor-spheroid-based anti-cancer-drug testing

Ibrahim G. Abd El-Sadek; Rion Morishita; Tomoko Mori; Shuichi Makita; Pradipta Mukherjee; Satoshi Matsusaka; Yoshiaki Yasuno

doi:10.1117/12.2649555

7 March 2023 Dynamic optical coherence tomography for tumor-spheroid-based anti-cancer-drug testing

Ibrahim G. Abd El-Sadek, Rion Morishita, Tomoko Mori, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, Yoshiaki Yasuno

Author Affiliations +

Proceedings Volume PC12378, Dynamics and Fluctuations in Biomedical Photonics XX; PC1237804 (2023) https://doi.org/10.1117/12.2649555
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We demonstrate OCT-based intracellular motility imaging method, so-called dynamic-OCT (D-OCT), and its application for tumor spheroid-based drug testing. The volumetric tomography is captured in 52.4 s using our custom-designed scanning protocol, which repeatedly capture 32 frames at each location in the tissue. Two algorithms including logarithmic intensity variance (LIV) and late OCT correlation decay speed (OCDSl) were used for tissue dynamics visualization. The utility of our proposed method is investigated for the comparison of three types of anti-cancer drugs applied to human breast cancer (MCF-7) spheroids. The drug type dependent alterations of cell morphology and viability have been successfully visualized.

Conference Presentation

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Ibrahim G. Abd El-Sadek, Rion Morishita, Tomoko Mori, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, and Yoshiaki Yasuno "Dynamic optical coherence tomography for tumor-spheroid-based anti-cancer-drug testing", Proc. SPIE PC12378, Dynamics and Fluctuations in Biomedical Photonics XX, PC1237804 (7 March 2023); https://doi.org/10.1117/12.2649555

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