All-optical neurophysiology using superfast polarization-sensitive off-axis full-field optical coherence microscopy (SPoOF OCM) at kilohertz frame rates

Rishyashring R. Iyer; Carlos A. Renteria; Jiho Kahng; Stephen A. Boppart

doi:10.1117/12.2649258

17 March 2023 All-optical neurophysiology using superfast polarization-sensitive off-axis full-field optical coherence microscopy (SPoOF OCM) at kilohertz frame rates

Rishyashring R. Iyer, Carlos A. Renteria, Jiho Kahng, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC12365, Neural Imaging and Sensing 2023; PC123650F (2023) https://doi.org/10.1117/12.2649258
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

One of the challenges in indirect measurements of electrical activity is its representation as biologically-relevant features. Non-invasive techniques for controlling neural activity, such as optogenetics with simultaneous optical imaging, have emerged as powerful and versatile tools. We demonstrated Superfast Polarization-sensitive Off-axis Full-field (SPoOF) OCM to image changes to both the optical phase and birefringence from the electrical activity of neurons at cellular resolution for an entire network at a millisecond scale. Here, we demonstrate all-optical neurophysiology with SPoOF OCM and optical excitation as a non-invasive versatile technique for studying neural circuits at high throughput, and a method to convert optical metrics to biologically relevant electrical features.

Conference Presentation

Citation Download Citation

Rishyashring R. Iyer, Carlos A. Renteria, Jiho Kahng, and Stephen A. Boppart "All-optical neurophysiology using superfast polarization-sensitive off-axis full-field optical coherence microscopy (SPoOF OCM) at kilohertz frame rates", Proc. SPIE PC12365, Neural Imaging and Sensing 2023, PC123650F (17 March 2023); https://doi.org/10.1117/12.2649258

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available