Quantitative imaging of glutathione using hyperspectral stimulated Raman scattering microscopy

Dinghuan Deng; Jianwei Chen; Tao Chen; Jin Wang; Meng C. Wang

doi:10.1117/12.2610245

2 March 2022 Quantitative imaging of glutathione using hyperspectral stimulated Raman scattering microscopy

Dinghuan Deng, Jianwei Chen, Tao Chen, Jin Wang, Meng C. Wang

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730Z (2022) https://doi.org/10.1117/12.2610245
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present a reversible reaction-based Raman probe for live-cell imaging of Glutathione (GSH) by the hyperspectral stimulated Raman scattering microscopy. By introducing a cyano group as the Raman reporter and an alkyne group as the internal standard, we have designed, synthesized, and characterized a series of Raman-based probes for ratiometric imaging and quantification of GSH levels in living cells and organisms.

Conference Presentation

Citation Download Citation

Dinghuan Deng, Jianwei Chen, Tao Chen, Jin Wang, and Meng C. Wang "Quantitative imaging of glutathione using hyperspectral stimulated Raman scattering microscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730Z (2 March 2022); https://doi.org/10.1117/12.2610245

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available