Autofluorescence lifetime of gelatin-methacrylate hydrogels is sensitive to changes in cross-linking and pH

Marcos R. Rodriguez; Kyle P. Quinn

doi:10.1117/12.2610121

2 March 2022 Autofluorescence lifetime of gelatin-methacrylate hydrogels is sensitive to changes in cross-linking and pH

Marcos R. Rodriguez, Kyle P. Quinn

Author Affiliations +

Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720I (2022) https://doi.org/10.1117/12.2610121
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Gelatin-methacrylate (GelMA) yields tunable hydrogels that are used in studies involving drug delivery and tissue engineering. Methods to validate their physicochemical properties however are typically destructive or require labeling, and there is a need for non-invasive techniques capable of evaluating dynamic changes in hydrogel properties. Here we demonstrate the sensitivity of label-free fluorescence lifetime imaging (FLIM) to alterations in GelMA cross-linking and pH levels. Increases in cross-linking shorten long-lifetime (τ2) species, while increases in pH shorten the short-lifetime (τ1) components of green autofluorescence. These findings demonstrate a non-destructive, label-free method with broad applicability in evaluating hydrogel properties.

Conference Presentation

Citation Download Citation

Marcos R. Rodriguez and Kyle P. Quinn "Autofluorescence lifetime of gelatin-methacrylate hydrogels is sensitive to changes in cross-linking and pH", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720I (2 March 2022); https://doi.org/10.1117/12.2610121

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