PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
We combine ultrasensitive microscopy with a novel, highly precise, and fast phase control to enable quantitative phase imaging of weakly scattering objects such as single microtubules. We demonstrate 3D mapping of microtubule networks and real-time 3D localization of single proteins labeled with small metallic nanoparticles. In particular, the 3D trajectories of microtubule-associated proteins acquired at the kHz rate revealed a complex trajectory of the protein diffusion on the surface of microtubules. The fast and accurate phase shaping technique combined with interferometric scattering microscopy pushes the limits of the quantitative phase imaging deep into the subdiffraction regime.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Hadrien M. L. Robert, Marek Piliarik, "Quantitative phase imaging at the nanoscale using interferometric scattering microscope and thermo-optic effect," Proc. SPIE PC11970, Quantitative Phase Imaging VIII, PC119700K (2 March 2022); https://doi.org/10.1117/12.2616899