Presentation
7 March 2022 Towards high-speed widefield ultraviolet photoacoustic remote sensing microscopy via voice-coil stage scanning and an on-demand pulsed laser
Author Affiliations +
Abstract
An estimated 20-40% of patients who undergo breast-conserving surgery require repeat operations due to the length of time required post-surgery to analyze the resected sample. Recently, a novel non-contact microscopy technique known as ultraviolet photoacoustic remote sensing (UV-PARS) has been developed, which can produce virtual hematoxylin and eosin (H&E) stained images. By using a voice-coil scanning stage in conjunction with an on-demand pulsed laser, we demonstrate 1cm x 1cm gross UV-PARS virtual H&E scans in approximately 50 seconds, along with full-resolution 1cm x 1cm scans in approximately 8 minutes. With widefield high-speed scanning, UV-PARS shows promise for future translation to clinical application.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Brendyn D. Cikaluk, Brendon S. Restall, Nathaniel J. M. Haven, Matthew T. Martell, and Roger J. Zemp "Towards high-speed widefield ultraviolet photoacoustic remote sensing microscopy via voice-coil stage scanning and an on-demand pulsed laser", Proc. SPIE PC11960, Photons Plus Ultrasound: Imaging and Sensing 2022, PC119602M (7 March 2022); https://doi.org/10.1117/12.2610286
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KEYWORDS
Pulsed laser operation

Ultraviolet radiation

Photoacoustic spectroscopy

Remote sensing

Absorption

Tissues

Modulation

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