Multi-plane 3D optical voltage imaging using high-speed multi-Z confocal microscopy

Timothy D. Weber; Maria V. Moya; Michael N. Economo; Jerome Mertz

doi:10.1117/12.2607867

28 April 2022 Multi-plane 3D optical voltage imaging using high-speed multi-Z confocal microscopy

Timothy D. Weber, Maria V. Moya, Michael N. Economo, Jerome Mertz

Proceedings Volume PC11946, Neural Imaging and Sensing 2022; PC1194606 (2022) https://doi.org/10.1117/12.2607867
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Fluorescent genetically encoded voltage indicators can be combined with optical imaging to provide high-throughput electrophysiologic recordings with single-spike resolution and subthreshold sensitivity. Such voltage imaging is highly demanding in terms of signal collection; thus, most experiments have been performed with widefield one-photon microscopy. Unfortunately, widefield techniques are susceptible to out-of-focus background and scattering, which degrades SNR, especially in high-density slice or in vivo experiments. In this work, we describe a multi-plane near-kHz-rate confocal microscope that effectively suppresses undesired background. This technique enables more densely labeled in vitro and in vivo imaging experiments, critical for the dissection of neural circuit dynamics.

Conference Presentation

Citation Download Citation

Timothy D. Weber, Maria V. Moya, Michael N. Economo, and Jerome Mertz "Multi-plane 3D optical voltage imaging using high-speed multi-Z confocal microscopy", Proc. SPIE PC11946, Neural Imaging and Sensing 2022, PC1194606 (28 April 2022); https://doi.org/10.1117/12.2607867

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