Label-free 3D refractive-index acquisition by micro-manipulations of cells in suspension
(Conference Presentation)

Natan T. Shaked

doi:10.1117/12.2209306

27 April 2016 Label-free 3D refractive-index acquisition by micro-manipulations of cells in suspension (Conference Presentation)

Natan T. Shaked

Author Affiliations +

Proceedings Volume 9718, Quantitative Phase Imaging II; 971804 (2016) https://doi.org/10.1117/12.2209306
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Our latest methods for non-invasive label-free acquisition of the three-dimensional (3-D) refractive-index maps of live cells in suspension are reviewed. These methods are based on the acquisition of off-axis interferograms of single or multiple cells in suspension from different angles using an external interferometric module, while fully rotating each cell using micro-manipulations. The interferometric projections are processed via computed tomographic phase microscopy reconstruction technique, which considers optical diffraction effects, into the 3-D refractive-index structure of the suspended cell. Till now, tomographic phase microscopy was obtained by acquiring a series of interferograms of the light transmitted through the sample in different angles by either using an entire sample rotation, or patch clamping a single cell, which is invasive to the cells, or alternatively, using various angles of illumination, which causes a limited acceptance angle, and an incomplete 3-D Fourier spectrum. In contrast, our methods allow fast acquisition with full angular range, and thus obtain an accurate 3-D refractive-index map of the imaged cell. By inspection of the 3-D refractive-index distribution of cells in suspension, the proposed methods can be useful for high-throughput, label-free characterization of biological processes and cellular transformations from healthy to pathological conditions.

Conference Presentation

Citation Download Citation

Natan T. Shaked "Label-free 3D refractive-index acquisition by micro-manipulations of cells in suspension (Conference Presentation)", Proc. SPIE 9718, Quantitative Phase Imaging II, 971804 (27 April 2016); https://doi.org/10.1117/12.2209306

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