Label-free imaging of developing vasculature in zebrafish with phase variance optical coherence microscopy

Yu Chen; Jeff Fingler; Le A. Trinh; Scott E. Fraser

doi:10.1117/12.2209653

9 March 2016 Label-free imaging of developing vasculature in zebrafish with phase variance optical coherence microscopy

Yu Chen, Jeff Fingler, Le A. Trinh, Scott E. Fraser

Proceedings Volume 9716, Optical Methods in Developmental Biology IV; 97160A (2016) https://doi.org/10.1117/12.2209653
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

A phase variance optical coherence microscope (pvOCM) has been created to visualize blood flow in the vasculature of zebrafish embryos, without using exogenous labels. The pvOCM imaging system has axial and lateral resolutions of 2 μm in tissue, and imaging depth of more than 100 μm. Imaging of 2–5 days post-fertilization zebrafish embryos identified the detailed structures of somites, spinal cord, gut and notochord based on intensity contrast. Visualization of the blood flow in the aorta, veins and intersegmental vessels was achieved with phase variance contrast. The pvOCM vasculature images were confirmed with corresponding fluorescence microscopy of a zebrafish transgene that labels the vasculature with green fluorescent protein. The pvOCM images also revealed functional information of the blood flow activities that is crucial for the study of vascular development.

Conference Presentation

Citation Download Citation

Yu Chen, Jeff Fingler, Le A. Trinh, and Scott E. Fraser "Label-free imaging of developing vasculature in zebrafish with phase variance optical coherence microscopy", Proc. SPIE 9716, Optical Methods in Developmental Biology IV, 97160A (9 March 2016); https://doi.org/10.1117/12.2209653

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