Novel plasmonic platform for ultra-sensitive detection and diagnostics

Sangram L. Raut; Ryan Rich; Tanya Shtoyko; Sebastian Requena; Ilkay Bora; Bo W. Laursen; Thomas Just Sørensen; Julian Borejdo; Zygmunt Gryczynski; Ignacy Gryczynski

doi:10.1117/12.2221923

1 March 2016 Novel plasmonic platform for ultra-sensitive detection and diagnostics

Sangram L. Raut, Ryan Rich, Tanya Shtoyko, Sebastian Requena, Ilkay Bora, Bo W. Laursen, Thomas Just Sørensen, Julian Borejdo, Zygmunt Gryczynski, Ignacy Gryczynski

Author Affiliations +

Proceedings Volume 9714, Single Molecule Spectroscopy and Superresolution Imaging IX; 971419 (2016) https://doi.org/10.1117/12.2221923
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.

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Sangram L. Raut, Ryan Rich, Tanya Shtoyko, Sebastian Requena, Ilkay Bora, Bo W. Laursen, Thomas Just Sørensen, Julian Borejdo, Zygmunt Gryczynski, and Ignacy Gryczynski "Novel plasmonic platform for ultra-sensitive detection and diagnostics", Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 971419 (1 March 2016); https://doi.org/10.1117/12.2221923

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