Tomographic phase microscopy using optical tweezers

Mor Habaza; Barak Gilboa; Yael Roichman; Natan T. Shaked

doi:10.1117/12.2183708

14 July 2015 Tomographic phase microscopy using optical tweezers

Mor Habaza, Barak Gilboa, Yael Roichman, Natan T. Shaked

Proceedings Volume 9536, Advanced Microscopy Techniques IV; and Neurophotonics II; 95360H (2015) https://doi.org/10.1117/12.2183708
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

We review our technique for tomographic phase microscopy with optical tweezers [1]. This tomographic phase microscopy approach enables full 3-D refractive-index reconstruction. Tomographic phase microscopy measures quantitatively the 3- D distribution of refractive-index in biological cells. We integrated our external interferometric module with holographic optical tweezers for obtaining quantitative phase maps of biological samples from a wide range of angles. The close-tocommon- path, off-axis interferometric system enables a full-rotation tomographic acquisition of a single cell using holographic optical tweezers for trapping and manipulating with a desired array of traps, while acquiring phase information of a single cell from all different angles and maintaining the native surrounding medium. We experimentally demonstrated two reconstruction algorithms: the filtered back-projection method and the Fourier diffraction method for 3-D refractive index imaging of yeast cells.

Citation Download Citation

Mor Habaza, Barak Gilboa, Yael Roichman, and Natan T. Shaked "Tomographic phase microscopy using optical tweezers", Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360H (14 July 2015); https://doi.org/10.1117/12.2183708

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