Plasmonic enhancement in the photoinactivation of Escherichia Coli using rose bengal and gold nanoparticles

Heike Kagel; Julia Honselmann Genannt Humme; Edvaldo Antonio Ribeiro Rosa; Rozane de Fátima Turchiello; Arandi Ginane Bezerra Junior

doi:10.1117/12.2180947

19 June 2015 Plasmonic enhancement in the photoinactivation of Escherichia Coli using rose bengal and gold nanoparticles

Heike Kagel, Julia Honselmann Genannt Humme, Edvaldo Antonio Ribeiro Rosa, Rozane de Fátima Turchiello, Arandi Ginane Bezerra Junior

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Proceedings Volume 9531, Biophotonics South America; 953138 (2015) https://doi.org/10.1117/12.2180947
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil

Abstract

In the present study we report on the ability of gold nanoparticles (AuNP) to enhance the antimicrobial activity of the photosensitizer Rose Bengal (RB), a very effective singlet oxygen generator. Our experiments were conducted using a suspension of Escherichia Coli in the presence of either RB or a combination of RB and AuNP. Nanoparticles were synthesized by laser ablation in water, which allows high purity, biologically friendly AuNP production, as compared to traditional chemical methods. Several relative concentrations of bacteria, photosensitizes and AuNP were studied. Bacterial survival rates were determined before and after LED light illumination. The phototoxicity of RB with and without AuNP was checked following illumination for 10 and 20 minutes. As a control, the dark toxicity of RB was verified. The results show that the survival rate of bacteria decreases significantly with the increase of RB concentration and illumination time, which is in accordance with previous works. Interestingly, our results also indicate a significant increase in the lethal photosensitization of RB in the presence of AuNP. We propose this effect is due to plasmonic light enhancement, considering the superposition of RB and AuNP absorption spectra, which favors electric field enhancement effects in the presence of AuNP. Similar experiments using the photosensitizer Methylene Blue (MB) allowed us to test our hypothesis for MB did not show any difference in its phototoxicity in the presence of AuNP. We propose this observed synergistic effect could be an effective way for improving photodynamic inactivation of microorganisms.

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Heike Kagel, Julia Honselmann Genannt Humme, Edvaldo Antonio Ribeiro Rosa, Rozane de Fátima Turchiello, and Arandi Ginane Bezerra Junior "Plasmonic enhancement in the photoinactivation of Escherichia Coli using rose bengal and gold nanoparticles", Proc. SPIE 9531, Biophotonics South America, 953138 (19 June 2015); https://doi.org/10.1117/12.2180947

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KEYWORDS

Nanoparticles

Bacteria

Laser ablation

Light emitting diodes

Plasmonics

Gold

Absorption

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