Optical detection of two-color-fluorophore barcode for nanopore DNA sensing

M. Zhang; I. Sychugov; T. Schmidt; J. Linnros

doi:10.1117/12.2179626

1 June 2015 Optical detection of two-color-fluorophore barcode for nanopore DNA sensing

M. Zhang, I. Sychugov, T. Schmidt, J. Linnros

Proceedings Volume 9519, Nanotechnology VII; 95190G (2015) https://doi.org/10.1117/12.2179626
Event: SPIE Microtechnologies, 2015, Barcelona, Spain

Abstract

A simple schematic on parallel optical detection of two-fluorophore barcode for single-molecule nanopore sensing is presented. The chosen two fluorophores, ATTO-532 and DY-521-XL, emitting in well-separated spectrum range can be excited at the same wavelength. A beam splitter was employed to separate signals from the two fluorophores and guide them to the same CCD camera. Based on a conventional microscope, sources of background in the nanopore sensing system, including membranes, compounds in buffer solution, and a detection cell was characterized. By photoluminescence excitation measurements, it turned out that silicon membrane has a negligible photoluminescence under the examined excitation from 440 nm to 560 nm, in comparison with a silicon nitrite membrane. Further, background signals from the detection cell were suppressed. Brownian motion of 450 bps DNA labelled with single ATTO-532 or DY-521-XL was successfully recorded by our optical system.

Citation Download Citation

M. Zhang, I. Sychugov, T. Schmidt, and J. Linnros "Optical detection of two-color-fluorophore barcode for nanopore DNA sensing", Proc. SPIE 9519, Nanotechnology VII, 95190G (1 June 2015); https://doi.org/10.1117/12.2179626

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KEYWORDS

Silicon

Luminescence

Microscopes

Signal detection

Molecules

Beam splitters

Glasses

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