Localised hydrogen peroxide sensing for reproductive health

Malcolm S. Purdey; Erik P. Schartner; Melanie L. Sutton-McDowall; Lesley J. Ritter; Jeremy Thompson; Tanya M. Monro; Andrew D. Abell

doi:10.1117/12.2178680

5 May 2015 Localised hydrogen peroxide sensing for reproductive health

Malcolm S. Purdey, Erik P. Schartner, Melanie L. Sutton-McDowall, Lesley J. Ritter, Jeremy Thompson, Tanya M. Monro, Andrew D. Abell

Author Affiliations +

Proceedings Volume 9506, Optical Sensors 2015; 950614 (2015) https://doi.org/10.1117/12.2178680
Event: SPIE Optics + Optoelectronics, 2015, Prague, Czech Republic

Abstract

The production of reactive oxygen species (ROS) is known to affect the developmental competence of embryos. Hydrogen peroxide (H₂O₂) an important reactive oxygen species, is also known to causes DNA damage and defective sperm function. Current techniques require incubating a developing embryo with an organic fluorophore which is potentially hazardous for the embryo. What we need is a localised ROS sensor which does not require fluorophores in solution and hence will allow continuous monitoring of H₂O₂ production without adversely affect the development of the embryo. Here we report studies on such a fibre-based sensor for the detection of H₂O₂ that uses a surface-bound aryl boronate fluorophore carboxyperoxyfluor-1(CPF1). Optical fibres present a unique platform due to desirable characteristics as dip sensors in biological solutions. Attempts to functionalise the fibre tips using polyelectrolyte layers and (3-aminopropyl)triethoxysilane (APTES) coatings resulted in a limited signal and poor fluorescent response to H₂O₂ due to a low tip surface density of the fluorophore. To increase the surface density, CPF1 was integrated into a polymer matrix formed on the fibre tip by a UV-catalysed polymerisation process of acrylamide onto a methacrylate silane layer. The polyacrylamide containing CPF1 gave a much higher surface density than previous surface attachment methods and the sensor was found to effectively detect H₂O₂. Using this method, biologically relevant concentrations of H₂O₂ were detected, enabling remote sensing studies into ROS releases from embryos throughout early development.

Citation Download Citation

Malcolm S. Purdey, Erik P. Schartner, Melanie L. Sutton-McDowall, Lesley J. Ritter, Jeremy Thompson, Tanya M. Monro, and Andrew D. Abell "Localised hydrogen peroxide sensing for reproductive health", Proc. SPIE 9506, Optical Sensors 2015, 950614 (5 May 2015); https://doi.org/10.1117/12.2178680

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available