Tissue fluorescence origins evaluation using excitation-emission matrices

A. Zhelyazkova; E. Borisova; L. Angelova; E. Pavlova; M. Keremedchiev; L. Avramov

doi:10.1117/12.2177795

8 January 2015 Tissue fluorescence origins evaluation using excitation-emission matrices

A. Zhelyazkova, E. Borisova, L. Angelova, E. Pavlova, M. Keremedchiev, L. Avramov

Proceedings Volume 9447, 18th International School on Quantum Electronics: Laser Physics and Applications; 944711 (2015) https://doi.org/10.1117/12.2177795
Event: Eighteenth International School on Quantum Electronics: Laser Physics and Applications, 2014, Sozopol, Bulgaria

Abstract

Autofluorescence has been proven to be a very sensitive, accurate, noninvasive method for detection of early pathological changes in tissues. This optical method has the potential to provide a real-time diagnosis of different benign, dysplastic and malignant tissue pathologies. We obtain tissue samples after surgical excision of preliminary clinically diagnosed tumours. Ethical approval for our investigations is received from Ethical Committee of University Hospital “Queen Jiovanna-ISUL” – Sofia, where the samples will be obtained as well. The investigations presented in this report are based on ex vivo measurements of excitation-emission matrices (EEM) for normal and neoplastic human tissue samples with various cutaneous malignant and dysplastic lesions, as well for gastrointestinal tract (GIT) normal mucosa, polyps and carcinoma. The origins of the endogenous fluorescence are found and the differences observed are discussed from the point of view of their diagnostic value and correlation with the morphological and biochemical changes occurred during the tumour development.

Citation Download Citation

A. Zhelyazkova, E. Borisova, L. Angelova, E. Pavlova, M. Keremedchiev, and L. Avramov "Tissue fluorescence origins evaluation using excitation-emission matrices", Proc. SPIE 9447, 18th International School on Quantum Electronics: Laser Physics and Applications, 944711 (8 January 2015); https://doi.org/10.1117/12.2177795

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