Multiplexed off-axis interferometric phase microscopy for dynamic cell measurements

Natan T. Shaked; Pinhas Girshovitz; Irena Frenklach

doi:10.1117/12.2078227

11 March 2015 Multiplexed off-axis interferometric phase microscopy for dynamic cell measurements

Natan T. Shaked, Pinhas Girshovitz, Irena Frenklach

Proceedings Volume 9336, Quantitative Phase Imaging; 93360B (2015) https://doi.org/10.1117/12.2078227
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

We present a new approach of optically multiplexing several off-axis interferograms on the same digital camera, each of which encodes a different field of view of the sample. Since the fringes of these interferograms are in different directions, as obtained experimentally by the optical system, we are able to double or even triple the amount of information that can be acquired in a single camera exposure, with the same number of camera pixels, while sharing the camera dynamic range. We show that this method can partially solve the problem of limited off-axis interferometric field of view due to low-coherence illumination. Our experimental demonstrations include quantitative phase imaging of microscopic diatom shells, fast swimming sperm cells and microorganisms, and contracting cardiomyocytes.

Citation Download Citation

Natan T. Shaked, Pinhas Girshovitz, and Irena Frenklach "Multiplexed off-axis interferometric phase microscopy for dynamic cell measurements", Proc. SPIE 9336, Quantitative Phase Imaging, 93360B (11 March 2015); https://doi.org/10.1117/12.2078227

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