Detecting the collagen-based hydrogels degradation by multiphoton microscopy (MPM)

Xuye Lang; Matthew J. Spousta; Julia G. Lyubovitsky

doi:10.1117/12.2080559

3 March 2015 Detecting the collagen-based hydrogels degradation by multiphoton microscopy (MPM)

Xuye Lang, Matthew J. Spousta, Julia G. Lyubovitsky

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93293V (2015) https://doi.org/10.1117/12.2080559
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Collagen is widely used in tissue engineering applications because of its biocompatibility and biodegradability. Detecting collagen microstructure can help to accelerate its applications in tissue engineering. In this study, we followed the changes in microstructure of collagen hydrogels that were digested with collagenase by MPM using Second Harmonic Generation (SHG) and Two photon Fluorescence (TPF) signals. The collagen hydrogels were modified by cross-linkers genipin, EDC or EDC+NHS. For unmodified collagen hydrogels, SHG images showed degradation was underway by about 20 min. For collagen hydrogels modified with EDC or EDC+NHS, preliminary data did not indicate obvious degradation after 22 hours. Modification with genipin induced new fibers that had TPF centered at about 490 nm and 600 nm. The SHG signals were weaker in genipin modified collagen hydrogels. TPF images illustrated that the degradation of the newly induced fluorescent fibers at the surface of the materials was underway after about 2 hours.

Citation Download Citation

Xuye Lang, Matthew J. Spousta, and Julia G. Lyubovitsky "Detecting the collagen-based hydrogels degradation by multiphoton microscopy (MPM)", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93293V (3 March 2015); https://doi.org/10.1117/12.2080559

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