High-resolution nonlinear ellipse rotation measurements for 3D microscopy

M. L. Miguez; E. C. Barbano; J. A. Coura; S. C. Zilio; L. Misoguti

doi:10.1117/12.2077819

5 March 2015 High-resolution nonlinear ellipse rotation measurements for 3D microscopy

M. L. Miguez, E. C. Barbano, J. A. Coura, S. C. Zilio, L. Misoguti

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93292L (2015) https://doi.org/10.1117/12.2077819
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Nonlinear optical effects have been widely explored for microscopy due to the possibility of three-dimension (3D) image acquisition. Harmonic generation and nonlinear absorption, for instance, were used for this purpose. Each nonlinear effect has its own characteristic, complexity, type of contrast, advantage and disadvantage, etc. Recently, we developed a new simple and sensitive method for measuring nonlinear ellipse rotation (NER) using a dual-phase lock-in amplifier, which could be successfully applied for measuring local nonlinearity distribution on a sample and, consequently, the image acquisition. The NER is a particular refractive nonlinear effect which appears when strong elliptical polarized laser beam propagates along one nonlinear material. It is type of refractive Kerr nonlinearity similar to self-focalization responsible for the signal in the Z-scan technique. The self-focalization is one of the most important refractive effects, but it cannot be used for image acquisition. On the other hand, NER does. Furthermore, such refractive nonlinearities signal can be very strong and serves as a new contrast for nonlinear microscopy.

Citation Download Citation

M. L. Miguez, E. C. Barbano, J. A. Coura, S. C. Zilio, and L. Misoguti "High-resolution nonlinear ellipse rotation measurements for 3D microscopy", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93292L (5 March 2015); https://doi.org/10.1117/12.2077819

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