Smartphone fluorescence spectroscopy

Hojoeng Yu; Yafang Tan; Brian T. Cunningham

doi:10.1117/12.2083999

2 March 2015 Smartphone fluorescence spectroscopy

Hojoeng Yu, Yafang Tan, Brian T. Cunningham

Proceedings Volume 9310, Frontiers in Biological Detection: From Nanosensors to Systems VII; 93100B (2015) https://doi.org/10.1117/12.2083999
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

We demonstrate the first use of smartphone spectrophotometry for readout of fluorescence-based biological assays. We evaluated the smartphone fluorimeter in the context of a fluorescent molecular beacon (MB) assay for detection of a specific nucleic acid sequences in a liquid test sample. The capability of distinguishing a one-point mismatch is also demonstrated by detecting single-base mutation in target nucleic acids. Our approach offers a route towards portable biomolecular assays for viral/bacterial pathogens, disease biomarkers, and toxins.

Citation Download Citation

Hojoeng Yu, Yafang Tan, and Brian T. Cunningham "Smartphone fluorescence spectroscopy", Proc. SPIE 9310, Frontiers in Biological Detection: From Nanosensors to Systems VII, 93100B (2 March 2015); https://doi.org/10.1117/12.2083999

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