Imaging microscopy by phase-contrast engine: retardation-modulated differential interference contrast microscope

Hiroshi Ishiwata; Masahide Itoh

doi:10.1117/12.2074378

5 November 2014 Imaging microscopy by phase-contrast engine: retardation-modulated differential interference contrast microscope

Hiroshi Ishiwata, Masahide Itoh

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Proceedings Volume 9272, Optical Design and Testing VI; 92720A (2014) https://doi.org/10.1117/12.2074378
Event: SPIE/COS Photonics Asia, 2014, Beijing, China

Abstract

In the field of biology and medicine, observation object of the microscope has been changing from the thin specimen to the thick living tissue. Furthermore, observation of the internal structure of a living tissue is also desired by low invasion. However, the real structure of a phase object with three-dimensional distribution such as a living tissue is difficult to observe, because of the influence of the phase distribution before and behind of observation position. We enabled observation of the internal structure of living tissue without stain, by adding a new function to reduce the influence of phase distribution to our Retardation-Modulated differential interference contrast (RM-DIC) microscope system.

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Hiroshi Ishiwata and Masahide Itoh "Imaging microscopy by phase-contrast engine: retardation-modulated differential interference contrast microscope", Proc. SPIE 9272, Optical Design and Testing VI, 92720A (5 November 2014); https://doi.org/10.1117/12.2074378

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KEYWORDS

Microscopes

Digital image correlation

Microscopy

Tissues

Modulation transfer functions

Luminescence

Phase transfer function

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