High speed fluorescence photoactivation localization microscopy imaging

Andrew J. Nelson; Mudalige S. Gunewardene; Samuel T. Hess

doi:10.1117/12.2064271

28 August 2014 High speed fluorescence photoactivation localization microscopy imaging

Andrew J. Nelson, Mudalige S. Gunewardene, Samuel T. Hess

Proceedings Volume 9169, Nanoimaging and Nanospectroscopy II; 91690P (2014) https://doi.org/10.1117/12.2064271
Event: SPIE NanoScience + Engineering, 2014, San Diego, California, United States

Abstract

Imaging live biological samples to study biomolecular dynamics requires a very high spatial and temporal resolution. Superresolution localization microscopy has allowed researchers to investigate biological systems whose sizes are below the diffraction limit (200-250 nm) using an optical microscope. Fluorescence Photoactivation Localization Microscopy (FPALM) and other localization microscopy techniques have recently been shown to be capable of rendering superresolution images obtained with acquisitions of shorter than 0.5 seconds. Here we will discuss the FPALM imaging technique, at both lower and higher imaging speeds. This talk will focus on the advantages, challenges, and drawbacks of high speed imaging localization microscopy.

Citation Download Citation

Andrew J. Nelson, Mudalige S. Gunewardene, and Samuel T. Hess "High speed fluorescence photoactivation localization microscopy imaging", Proc. SPIE 9169, Nanoimaging and Nanospectroscopy II, 91690P (28 August 2014); https://doi.org/10.1117/12.2064271

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