Plasmonic enhanced pulsed laser induced optoporation and transfection of cells (presentation video)

Michel Meunier

doi:10.1117/12.2063896

17 August 2014 Plasmonic enhanced pulsed laser induced optoporation and transfection of cells (presentation video)

Michel Meunier

Proceedings Volume 9166, Biosensing and Nanomedicine VII; 916609 (2014) https://doi.org/10.1117/12.2063896
Event: SPIE NanoScience + Engineering, 2014, San Diego, California, United States

Abstract

A new technique is introduced to perform optoporation and transfection of living cells using a laser and nanotechnology. Irradiating plasmonics nanostructures by an ultrafast laser beam produces highly localised processes on the nanoscale in the biological surrounding medium, yielding to the optoporation of the cell membrane.. These nanoparticles could be functionalised to target specific biological entities, thus performing multiple targeted processes on the nanoscale. . We are able to perform gene transfection in living cell with an optoporation efficiency as high as 70%. Complete physical model was developed to determine the basic mechanism underlying this new process. Our laser technology shows promises as an innovative tool for fundamental research in biology and medicine as well as an efficient alternative nanosurgery technology that could be adapted to therapeutic tools in the clinic.

Citation Download Citation

Michel Meunier "Plasmonic enhanced pulsed laser induced optoporation and transfection of cells (presentation video)", Proc. SPIE 9166, Biosensing and Nanomedicine VII, 916609 (17 August 2014); https://doi.org/10.1117/12.2063896

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