Numerical focusing in digital holographic microscopy with partially spatially coherent illumination in transmission

Anton A. Grebenyuk; Vladimir P. Ryabukho

doi:10.1117/12.2052837

30 January 2014 Numerical focusing in digital holographic microscopy with partially spatially coherent illumination in transmission

Anton A. Grebenyuk, Vladimir P. Ryabukho

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Proceedings Volume 9031, Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV; 903119 (2014) https://doi.org/10.1117/12.2052837
Event: Saratov Fall Meeting 2013, 2013, Saratov, Russian Federation

Abstract

Digital holographic microscopy (DHM) combines the possibility of high resolution quantitative phase imaging with numerical focusing and in transmission mode is widely used for live cell imaging. The use of partially spatially coherent illumination in DHM provides some advantages over the conventional spatially coherent illumination, but modifies the signal properties and requires detailed investigation, especially in the part of numerical focusing. In this paper we present theoretical analysis of the effects of partially spatially coherent illumination in transmission-mode DHM on its impulse response and numerical focusing possibilities. Further, we propose a method of providing high quality impulse response (as by the spatially low-coherent illumination), preserving large maximum distance of numerical focusing.

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Anton A. Grebenyuk and Vladimir P. Ryabukho "Numerical focusing in digital holographic microscopy with partially spatially coherent illumination in transmission", Proc. SPIE 9031, Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV, 903119 (30 January 2014); https://doi.org/10.1117/12.2052837

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