Near infrared fluorescence quenching properties of copper (II) ions for potential applications in biological imaging

Dolonchampa Maji; Mingzhou Zhou; Pinaki Sarder; Samuel Achilefu

doi:10.1117/12.2053746

7 March 2014 Near infrared fluorescence quenching properties of copper (II) ions for potential applications in biological imaging

Dolonchampa Maji, Mingzhou Zhou, Pinaki Sarder, Samuel Achilefu

Author Affiliations +

Proceedings Volume 8956, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VI; 89560K (2014) https://doi.org/10.1117/12.2053746
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Fluorescence quenching properties of copper(II) ions have been used for designing Cu(II) sensitive fluorescent molecular probes. In this paper, we demonstrate that static quenching plays a key role in free Cu(II)-mediated fluorescence quenching of a near infrared (NIR) fluorescent dye cypate. The Stern-Volmer quenching constant was calculated to be K_SV = 970,000 M^-1 in 25 mM MES buffer, pH 6.5 at room temperature. We synthesized LS835, a compound containing cypate attached covalently to chelated Cu(II) to study fluorescence quenching by chelated Cu(II). The fluorescence quenching mechanism of chelated Cu(II) is predominantly dynamic or collisional quenching. The quenching efficiency of chelated Cu(II) was calculated to be 58% ± 6% in dimethylsulfoxide at room temperature. Future work will involve further characterization of the mechanism of NIR fluorescence quenching by Cu(II) and testing its reversibility for potential applications in designing fluorophore-quencher based molecular probes for biological imaging.

Citation Download Citation

Dolonchampa Maji, Mingzhou Zhou, Pinaki Sarder, and Samuel Achilefu "Near infrared fluorescence quenching properties of copper (II) ions for potential applications in biological imaging", Proc. SPIE 8956, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VI, 89560K (7 March 2014); https://doi.org/10.1117/12.2053746

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