Imaging mesenchymal stem cells containing single wall nanotube nanoprobes in a 3D scaffold using photo-thermal optical coherence tomography

Emma Connolly; Hrebesh M. Subhash; Martin Leahy; Niall Rooney; Frank Barry; Mary Murphy; Valerie Barron

doi:10.1117/12.2039829

20 February 2014 Imaging mesenchymal stem cells containing single wall nanotube nanoprobes in a 3D scaffold using photo-thermal optical coherence tomography

Emma Connolly, Hrebesh M. Subhash, Martin Leahy, Niall Rooney, Frank Barry, Mary Murphy, Valerie Barron

Author Affiliations +

Proceedings Volume 8954, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI; 89540W (2014) https://doi.org/10.1117/12.2039829
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Despite the fact, that a range of clinically viable imaging modalities, such as magnetic resonance imaging (MRI), computed tomography (CT), photo emission tomography (PET), ultrasound and bioluminescence imaging are being optimised to track cells in vivo, many of these techniques are subject to limitations such as the levels of contrast agent required, toxic effects of radiotracers, photo attenuation of tissue and backscatter. With the advent of nanotechnology, nanoprobes are leading the charge to overcome these limitations. In particular, single wall nanotubes (SWNT) have been shown to be taken up by cells and as such are effective nanoprobes for cell imaging. Consequently, the main aim of this research is to employ mesenchymal stem cells (MSC) containing SWNT nanoprobes to image cell distribution in a 3D scaffold for cartilage repair. To this end, MSC were cultured in the presence of 32μg/ml SWNT in cell culture medium (αMEM, 10% FBS, 1% penicillin/streptomycin) for 24 hours. Upon confirmation of cell viability, the MSC containing SWNT were encapsulated in hyaluronic acid gels and loaded on polylactic acid polycaprolactone scaffolds. After 28 days in complete chondrogenic medium, with medium changes every 2 days, chondrogenesis was confirmed by the presence of glycosaminoglycan. Moreover, using photothermal optical coherence tomography (PT-OCT), the cells were seen to be distributed through the scaffold with high resolution. In summary, these data reveal that MSC containing SWNT nanoprobes in combination with PT-OCT offer an exciting opportunity for stem cell tracking in vitro for assessing seeding scaffolds and in vivo for determining biodistribution.

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Emma Connolly, Hrebesh M. Subhash, Martin Leahy, Niall Rooney, Frank Barry, Mary Murphy, and Valerie Barron "Imaging mesenchymal stem cells containing single wall nanotube nanoprobes in a 3D scaffold using photo-thermal optical coherence tomography", Proc. SPIE 8954, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI, 89540W (20 February 2014); https://doi.org/10.1117/12.2039829

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KEYWORDS

Single walled carbon nanotubes

Nanoprobes

Optical coherence tomography

Imaging systems

3D image processing

Stem cells

Tissues

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