Live cell imaging based on surface plasmon-enhanced fluorescence microscopy using random nanostructures

Youngjin Oh; Wonju Lee; Taehwang Son; Sook Young Kim; Jeon-Soo Shin; Donghyun Kim

doi:10.1117/12.2038868

20 February 2014 Live cell imaging based on surface plasmon-enhanced fluorescence microscopy using random nanostructures

Youngjin Oh, Wonju Lee, Taehwang Son, Sook Young Kim, Jeon-Soo Shin, Donghyun Kim

Author Affiliations +

Proceedings Volume 8954, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI; 89540E (2014) https://doi.org/10.1117/12.2038868
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Localized surface plasmon enhanced microscopy based on nanoislands of random spatial distribution was demonstrated for imaging live cells and molecular interactions. Nanoislands were produced without lithography by high temperature annealing under various processing conditions. The localization of near-field distribution that is associated with localized surface plasmon on metallic random nanoislands was analyzed theoretically and experimentally in comparison with periodic nanostructures. For experimental validation in live cell imaging, mouse macrophage-like cell line stained with Alexa Fluor 488 was prepared on nanoislands. The results suggest the possibility of attaining the imaging resolution on the order of 80 nm.

Citation Download Citation

Youngjin Oh, Wonju Lee, Taehwang Son, Sook Young Kim, Jeon-Soo Shin, and Donghyun Kim "Live cell imaging based on surface plasmon-enhanced fluorescence microscopy using random nanostructures", Proc. SPIE 8954, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI, 89540E (20 February 2014); https://doi.org/10.1117/12.2038868

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