PEGylation of Concanavalin A to decrease nonspecific interactions in a fluorescent glucose sensor

Alexander A. Abraham; Brian M. Cummins; Andrea K. Locke; Melissa A. Grunlan; Gerard L. Coté

doi:10.1117/12.2040457

28 February 2014 PEGylation of Concanavalin A to decrease nonspecific interactions in a fluorescent glucose sensor

Alexander A. Abraham, Brian M. Cummins, Andrea K. Locke, Melissa A. Grunlan, Gerard L. Coté

Proceedings Volume 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics; 895112 (2014) https://doi.org/10.1117/12.2040457
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The ability of people with diabetes to both monitor and regulate blood sugar levels is limited by the conventional “finger-prick” test that provides intermittent, single point measurements. Toward the development of a continuous glucose monitoring (CGM) system, the lectin, Concanavalin A (ConA), has been utilized as a component in a Förster resonance energy transfer (FRET), competitive glucose binding assay. Recently, to avoid reversibility problems associated with ConA aggregation, a suitable competing ligand labeled with 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt (APTS) has been engineered. However, its ability to function as part of a glucose sensing assay is compromised due to the negative charge (at physiological pH) of native ConA that gives rise to non-specific binding with other ConA groups as well as with electrostatically charged assay-delivery carriers. To minimize these undesirable interactions, we have conjugated ConA with monomethoxy-poly(ethylene glycol) (mPEG) (i.e. “PEGylation”). In this preliminary research, fluorescently-labeled ConA was successfully PEGylated with mPEG-Nhydroxylsuccinimide( succinimidyl carbonate) (mPEG-NHS(SC)). The FRET response of APTS-labeled competing ligand (donor) conveyed an increase in the fluorescence intensity with increasing glucose concentrations.

Citation Download Citation

Alexander A. Abraham, Brian M. Cummins, Andrea K. Locke, Melissa A. Grunlan, and Gerard L. Coté "PEGylation of Concanavalin A to decrease nonspecific interactions in a fluorescent glucose sensor", Proc. SPIE 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics, 895112 (28 February 2014); https://doi.org/10.1117/12.2040457

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Glucose

Fluorescence resonance energy transfer

Luminescence

Blood

Magnesium

Sodium

Carbonates

Show All Keywords

Keywords/Phrases

Search In:

Publication Years