In vivo and in vitro hyperspectral imaging of cervical neoplasia

Chaojian Wang; Wenli Zheng; Yanggao Bu; Shufang Chang; Qingping Tong; Shiwu Zhang; Ronald X. Xu

doi:10.1117/12.2041046

28 February 2014 In vivo and in vitro hyperspectral imaging of cervical neoplasia

Chaojian Wang, Wenli Zheng, Yanggao Bu, Shufang Chang, Qingping Tong, Shiwu Zhang, Ronald X. Xu

Proceedings Volume 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics; 89510M (2014) https://doi.org/10.1117/12.2041046
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Cervical cancer is a prevalent disease in many developing countries. Colposcopy is the most common approach for screening cervical intraepithelial neoplasia (CIN). However, its clinical efficacy heavily relies on the examiner’s experience. Spectroscopy is a potentially effective method for noninvasive diagnosis of cervical neoplasia. In this paper, we introduce a hyperspectral imaging technique for noninvasive detection and quantitative analysis of cervical neoplasia. A hyperspectral camera is used to collect the reflectance images of the entire cervix under xenon lamp illumination, followed by standard colposcopy examination and cervical tissue biopsy at both normal and abnormal sites in different quadrants. The collected reflectance data are calibrated and the hyperspectral signals are extracted. Further spectral analysis and image processing works are carried out to classify tissue into different types based on the spectral characteristics at different stages of cervical intraepithelial neoplasia. The hyperspectral camera is also coupled with a lab microscope to acquire the hyperspectral transmittance images of the pathological slides. The in vivo and the in vitro imaging results are compared with clinical findings to assess the accuracy and efficacy of the method.

Citation Download Citation

Chaojian Wang, Wenli Zheng, Yanggao Bu, Shufang Chang, Qingping Tong, Shiwu Zhang, and Ronald X. Xu "In vivo and in vitro hyperspectral imaging of cervical neoplasia", Proc. SPIE 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics, 89510M (28 February 2014); https://doi.org/10.1117/12.2041046

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