Fluorescence lifetime imaging of induced pluripotent stem cells

Aisada Uchugonova; Ana Batista; Karsten König

doi:10.1117/12.2037662

28 February 2014 Fluorescence lifetime imaging of induced pluripotent stem cells

Aisada Uchugonova, Ana Batista, Karsten König

Proceedings Volume 8948, Multiphoton Microscopy in the Biomedical Sciences XIV; 89481I (2014) https://doi.org/10.1117/12.2037662
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The multiphoton FLIM tomograph MPTflex with its flexible scan head, articulated arm, and the tunable femtosecond laser source was employed to study cell monolayers and 3D cell clusters. FLIM was performed with 250 ps temporal resolution and submicron special resolution using time-correlated single photon counting. The autofluorescence based on NAD(P)H and flavins/flavoproteins has been measured in mouse embryonic fibroblasts, induced pluripotent stem cells (iPS cells) originated from mouse embryonic fibroblasts and non-proliferative mouse embryonic fibroblasts.

Citation Download Citation

Aisada Uchugonova, Ana Batista, and Karsten König "Fluorescence lifetime imaging of induced pluripotent stem cells", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89481I (28 February 2014); https://doi.org/10.1117/12.2037662

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