Monitoring the influence of  antibiotic exposure using Raman spectroscopy

Ota Samek; Pavel Zemanek; Silvie Bernatova; Jan Jezek; Mojmir Sery; Petr Jakl; Martin Siler; Filip Ruzicka

doi:10.1117/12.2038595

4 March 2014 Monitoring the influence of antibiotic exposure using Raman spectroscopy

Ota Samek, Pavel Zemanek, Silvie Bernatova, Jan Jezek, Mojmir Sery, Petr Jakl, Martin Siler, Filip Ruzicka

Proceedings Volume 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry; 89390P (2014) https://doi.org/10.1117/12.2038595
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Here we report on combination of the data obtained from MICs (minimum inhibitory concentrations) with infor- mation of microoragnisms fingerprint provided by Raman spectroscopy. In our feasibility study we could follow mechanisms of the bacteriostatic versus bactericidal action on biofilm-positive Staphylococcus epidermidis simply by monitoring Raman bands corresponding to DNA translating the changes introduced by selected antibiotics. The Raman spectra of Staphylococcus epidermidis treated with a bacteriostatic agent show little effect on DNA which is in contrast with the action of a bactericidal agent where decreased in dedicated Raman spectra signal strength suggests DNA fragmentation. Moreover, we demonstrate that Raman tweezers are indeed able to distinguish strains of biofilm-forming (biofilm-positive) and biofilm-negative Staphylococcus epidermidis strains using principal component analysis (PCA).

Citation Download Citation

Ota Samek, Pavel Zemanek, Silvie Bernatova, Jan Jezek, Mojmir Sery, Petr Jakl, Martin Siler, and Filip Ruzicka "Monitoring the influence of antibiotic exposure using Raman spectroscopy", Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390P (4 March 2014); https://doi.org/10.1117/12.2038595

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