Raman spectroscopy and oral exfoliative cytology

Aditi Sahu; Nupur Shah; Manoj Mahimkar; Mandavi Garud; Sandeep Pagare; Sudhir Nair; C. Murali Krishna

doi:10.1117/12.2052794

4 March 2014 Raman spectroscopy and oral exfoliative cytology

Aditi Sahu, Nupur Shah, Manoj Mahimkar, Mandavi Garud, Sandeep Pagare, Sudhir Nair, C. Murali Krishna

Proceedings Volume 8926, Photonic Therapeutics and Diagnostics X; 89262N (2014) https://doi.org/10.1117/12.2052794
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Early detection of oral cancers can substantially improve disease-free survival rates. Ex vivo and in vivo Raman spectroscopic (RS) studies on oral cancer have demonstrated the applicability of RS in identifying not only malignant and premalignant conditions but also cancer-field-effects: the earliest events in oral carcinogenesis. RS has also been explored for cervical exfoliated cells analysis. Exfoliated cells are associated with several advantages like non-invasive sampling, higher patient compliance, transportation and analysis at a central facility: obviating need for on-site instrumentation. Thus, oral exfoliative cytology coupled with RS may serve as a useful adjunct for oral cancer screening. In this study, exfoliated cells from healthy controls with and without tobacco habits, premalignant lesions (leukoplakia and tobacco-pouch-keratosis) and their contralateral mucosa were collected using a Cytobrush. Cells were harvested by vortexing and centrifugation at 6000 rpm. The cellular yield was ascertained using Neubauer’s chamber. Cell pellets were placed on a CaF₂ window and Raman spectra were acquired using a Raman microprobe (40X objective) coupled HE-785 Raman spectrometer. Approximately 7 spectra were recorded from each pellet, following which pellet was smeared onto a glass slide, fixed in 95% ethanol and subjected to Pap staining for cytological diagnosis (gold standard). Preliminary PC-LDA followed by leave-one-out cross validation indicate delineation of cells from healthy and all pathological conditions. A tendency of classification was also seen between cells from contralateral, healthy tobacco and site of premalignant lesions. These results will be validated by cytological findings, which will serve as the basis for building standard models of each condition.

Citation Download Citation

Aditi Sahu, Nupur Shah, Manoj Mahimkar, Mandavi Garud, Sandeep Pagare, Sudhir Nair, and C. Murali Krishna "Raman spectroscopy and oral exfoliative cytology", Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89262N (4 March 2014); https://doi.org/10.1117/12.2052794

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available