Green synthesis of silver nanoparticles as antibacterial agent using Rhodomyrtus tomentosa acetone extract

Supayang Piyawan Voravuthikunchai; Julalak Chorachoo; Lily Jaiswal; Shiv Shankar

doi:10.1117/12.2035591

7 December 2013 Green synthesis of silver nanoparticles as antibacterial agent using Rhodomyrtus tomentosa acetone extract

Supayang Piyawan Voravuthikunchai, Julalak Chorachoo, Lily Jaiswal, Shiv Shankar

Author Affiliations +

Proceedings Volume 8923, Micro/Nano Materials, Devices, and Systems; 89235J (2013) https://doi.org/10.1117/12.2035591
Event: SPIE Micro+Nano Materials, Devices, and Applications, 2013, Melbourne, Victoria, Australia

Abstract

The capability of Rhodomyrtus tomentosa acetone extract (RAE) for the production of silver nanoparticles (AgNPs) has been explored for the first time. Silver nanoparticles with a surface plasmon resonance band centered at 420-430 nm were synthesized by reacting RAE with AgNO3. Reaction time, temperature, concentration of AgNO3 and RAE could accelerate the reduction rate of Ag⁺ and affect AgNPs size. The nanoparticles were found to be 10-30 nm in size and spherical in shape. XRD data demonstrated crystalline nature of AgNPs dominated by (200) facets. FTIR results showed decrease in intensity of peaks at 3394, 1716 and 1618 cm^-1 indicating the involvement of O-H, carbonyl group and C=C stretching with the formation of AgNPs with RAE, respectively. The C-O-C and C-N stretching suggested the presence of many phytochemicals on the surface of the nanoparticles. High negative zeta potential values confirmed the stability of AgNPs in water. In vitro antibacterial activity of AgNPs was tested against Staphylococcus aureus using broth microdilution method. AgNPs capped with RAE demonstrated profound antibacterial activity against the organisms with minimum inhibitory concentration and minimum bactericidal concentration in the range between 3.1-6.2 and 6.2-50 μgmL^-1, respectively. The synthesized nanoparticles could be applied as an effective antimicrobial agent against staphylococcal infections.

Citation Download Citation

Supayang Piyawan Voravuthikunchai, Julalak Chorachoo, Lily Jaiswal, and Shiv Shankar "Green synthesis of silver nanoparticles as antibacterial agent using Rhodomyrtus tomentosa acetone extract", Proc. SPIE 8923, Micro/Nano Materials, Devices, and Systems, 89235J (7 December 2013); https://doi.org/10.1117/12.2035591

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KEYWORDS

Nanoparticles

Silver

Absorbance

FT-IR spectroscopy

Spectrophotometry

Absorption

Crystals

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