A microfluidic platform to study the mechano sensational properties of ion channels

Sara Baratchi; Francisco J. Tovar-Lopez; Khashayar Khoshmanesh; Megan Grace; William Darby; Peter McIntyre; Arnan Mitchell

doi:10.1117/12.2033734

7 December 2013 A microfluidic platform to study the mechano sensational properties of ion channels

Sara Baratchi, Francisco J. Tovar-Lopez, Khashayar Khoshmanesh, Megan Grace, William Darby, Peter McIntyre, Arnan Mitchell

Author Affiliations +

Proceedings Volume 8923, Micro/Nano Materials, Devices, and Systems; 89232C (2013) https://doi.org/10.1117/12.2033734
Event: SPIE Micro+Nano Materials, Devices, and Applications, 2013, Melbourne, Victoria, Australia

Abstract

Microfluidic platforms have been widely considered as an enabling technology for studying the ion transport phenomena of cells under precisely controlled shear stresses. Here, we report the application of a unique microfluidic platform to analyze the response of transgenic TRPV4-HEK293 cells in response to different shear stresses and in one field of view. Applying this system, we show the kinetics of calcium signalling at different shear stresses in TRPV4 positive cells and elucidate the threshold of their response. We show that there is direct correlation between the magnitude of shear stress and percentage of cells that are able to sense that level of shear. Further, we show that shear stress-induced elevation in intracellular calcium levels ([Ca²⁺]i) is through calcium influx from extracellular sources. The results demonstrate that the microfluidic system has unique capabilities for analysis of shear stress on adhesive cells and that it should be amenable to moderate throughput applications.

Citation Download Citation

Sara Baratchi, Francisco J. Tovar-Lopez, Khashayar Khoshmanesh, Megan Grace, William Darby, Peter McIntyre, and Arnan Mitchell "A microfluidic platform to study the mechano sensational properties of ion channels", Proc. SPIE 8923, Micro/Nano Materials, Devices, and Systems, 89232C (7 December 2013); https://doi.org/10.1117/12.2033734

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Ion channels

Microfluidics

Calcium

Biological research

Proteins

Ions

Microscopes

Show All Keywords

Keywords/Phrases

Search In:

Publication Years