Orientational tomography of optical axes directions distributions of multilayer biological tissues birefringent polycrystalline networks

Natalia I. Zabolotna; Rostyslav Y. Dovhaliuk

doi:10.1117/12.2021462

27 September 2013 Orientational tomography of optical axes directions distributions of multilayer biological tissues birefringent polycrystalline networks

Natalia I. Zabolotna, Rostyslav Y. Dovhaliuk

Author Affiliations +

Proceedings Volume 8873, Polarization Science and Remote Sensing VI; 887313 (2013) https://doi.org/10.1117/12.2021462
Event: SPIE Optical Engineering + Applications, 2013, San Diego, California, United States

Abstract

We present a novel measurement method of optic axes orientation distribution which uses a relatively simple measurement setup. The principal difference of our method from other well-known methods lies in direct approach for measuring the orientation of optical axis of polycrystalline networks biological crystals. Our test polarimetry setup consists of HeNe laser, quarter wave plate, two linear polarizers and a CCD camera. We also propose a methodology for processing of measured optic axes orientation distribution which consists of evaluation of statistical, correlational and spectral moments. Such processing of obtained data can be used to classify particular tissue sample as “healthy” or “pathological”. For our experiment we use thin layers of histological section of normal and muscular dystrophy tissue sections. It is shown that the difference between mentioned moments` values of normal and pathological samples can be quite noticeable with relative difference up to 6.26.

Citation Download Citation

Natalia I. Zabolotna and Rostyslav Y. Dovhaliuk "Orientational tomography of optical axes directions distributions of multilayer biological tissues birefringent polycrystalline networks", Proc. SPIE 8873, Polarization Science and Remote Sensing VI, 887313 (27 September 2013); https://doi.org/10.1117/12.2021462

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