Evaluating cell matrix mechanics using an integrated nonlinear optical tweezer-confocal imaging system

Berney Peng; Carlo A. C. Alonzo; Lawrence Xia; Lucia Speroni; Irene Georgakoudi; Ana M. Soto; Carlos Sonnenschein; Mark Cronin-Golomb

doi:10.1117/12.2027155

12 September 2013 Evaluating cell matrix mechanics using an integrated nonlinear optical tweezer-confocal imaging system

Berney Peng, Carlo A. C. Alonzo, Lawrence Xia, Lucia Speroni, Irene Georgakoudi, Ana M. Soto, Carlos Sonnenschein, Mark Cronin-Golomb

Author Affiliations +

Proceedings Volume 8810, Optical Trapping and Optical Micromanipulation X; 88101O (2013) https://doi.org/10.1117/12.2027155
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

Biomechanics plays a central role in breast epithelial morphogenesis. In this study we have used 3D cultures in which normal breast epithelial cells are able to organize into rounded acini and tubular ducts, the main structures found in the breast tissue. We have identified fiber organization as a main determinant of ductal organization. While bulk rheological properties of the matrix seem to play a negligible role in determining the proportion of acini versus ducts, local changes may be pivotal in shape determination. As such, the ability to make microscale rheology measurements coupled with simultaneous optical imaging in 3D cultures can be critical to assess the biomechanical factors underlying epithelial morphogenesis. This paper describes the inclusion of optical tweezers based microrheology in a microscope that had been designed for nonlinear optical imaging of collagen networks in ECM. We propose two microrheology methods and show preliminary results using a gelatin hydrogel and collagen/Matrigel 3D cultures containing mammary gland epithelial cells.

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Berney Peng, Carlo A. C. Alonzo, Lawrence Xia, Lucia Speroni, Irene Georgakoudi, Ana M. Soto, Carlos Sonnenschein, and Mark Cronin-Golomb "Evaluating cell matrix mechanics using an integrated nonlinear optical tweezer-confocal imaging system", Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 88101O (12 September 2013); https://doi.org/10.1117/12.2027155

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KEYWORDS

Collagen

Breast

Optical spheres

3D image processing

Optical tweezers

Second-harmonic generation

Beam splitters

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