Wavefront coding using a spatial light modulator for extended depth of field microscopy

Michael Gierlak; Stephanie Albrecht; Josef Kauer; Eric Leverenz; Ingeborg E. Beckers

doi:10.1117/12.2032618

18 June 2013 Wavefront coding using a spatial light modulator for extended depth of field microscopy

Michael Gierlak, Stephanie Albrecht, Josef Kauer, Eric Leverenz, Ingeborg E. Beckers

Proceedings Volume 8798, Clinical and Biomedical Spectroscopy and Imaging III; 879803 (2013) https://doi.org/10.1117/12.2032618
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

Pupil phase masks for enhanced depth of field microscopy were investigated by using a spatial light modulator. The phase masks were evaluated with simulations in terms of the mean square error between in-focus and out-of-focus point spread functions. The resulting best-performing phase masks were tested for fluorosphere samples using a microscope add-on containing the SLM. First, z-stacks of fixed fluorospheres in an agarose medium were recorded in order to measure the extended depth of field. The same measurements were also performed on fluorospheres subjected to Brownian motion in an aqueous solution. The results show that with deconvolution and appropriate filtering it is possible to obtain sharp fluorosphere images with an extended depth of field of at least 10 μm.

Citation Download Citation

Michael Gierlak, Stephanie Albrecht, Josef Kauer, Eric Leverenz, and Ingeborg E. Beckers "Wavefront coding using a spatial light modulator for extended depth of field microscopy", Proc. SPIE 8798, Clinical and Biomedical Spectroscopy and Imaging III, 879803 (18 June 2013); https://doi.org/10.1117/12.2032618

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