Techniques for 3D tracking of single molecules with nanometer accuracy in living cells

Lucia Gardini; Marco Capitanio; Francesco Saverio Pavone

doi:10.1117/12.2032502

17 June 2013 Techniques for 3D tracking of single molecules with nanometer accuracy in living cells

Lucia Gardini, Marco Capitanio, Francesco Saverio Pavone

Proceedings Volume 8797, Advanced Microscopy Techniques III; 879703 (2013) https://doi.org/10.1117/12.2032502
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

We describe a microscopy technique that, combining wide-field single molecule microscopy, bifocal imaging and Highly Inclined and Laminated Optical sheet (HILO) microscopy, allows a 3D tracking with nanometer accuracy of single fluorescent molecules in vitro and in living cells.

Citation Download Citation

Lucia Gardini, Marco Capitanio, and Francesco Saverio Pavone "Techniques for 3D tracking of single molecules with nanometer accuracy in living cells", Proc. SPIE 8797, Advanced Microscopy Techniques III, 879703 (17 June 2013); https://doi.org/10.1117/12.2032502

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