Label-free biochemical characterization of bovine sperm cells using Raman microscopy

A. C. De Luca; S. Manago; M. A. Ferrara; L. Sirleto; R. Puglisi; D. Balduzzi; A. Galli; I. Rendina; P. Ferraro; G. Coppola

doi:10.1117/12.2021575

23 May 2013 Label-free biochemical characterization of bovine sperm cells using Raman microscopy

A. C. De Luca, S. Manago, M. A. Ferrara, L. Sirleto, R. Puglisi , D. Balduzzi, A. Galli, I. Rendina, P. Ferraro, G. Coppola

Author Affiliations +

Proceedings Volume 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials; 87920E (2013) https://doi.org/10.1117/12.2021575
Event: SPIE Optical Metrology 2013, 2013, Munich, Germany

Abstract

Raman spectroscopy is a label-free and non-invasive method that measures the inelastic scattered light from a sample giving insight into the vibration eigenmodes of the excited molecules. For these reasons, Raman spectroscopy has been used as a powerful tool to investigate different biological tissues and living cells. In this paper, we present a Raman spectroscopy-based method for sensitive biochemical characterization of bovine sperm cells. Importantly, by analysing separate Raman spectra from the nucleus, acrosomale vesicle and tail of single sperm cells, we are able to identify characteristic Raman features associated with DNA, protein and lipid molecular vibrations for discriminating among different locations inside the cell with sub-micrometric resolution (∼0.3 μm). We demonstrate that our Raman spectroscopy facilitates spectral assignment and increases detection sensitivity, opening the way for novel bio-imaging platforms.

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A. C. De Luca, S. Manago, M. A. Ferrara, L. Sirleto, R. Puglisi , D. Balduzzi, A. Galli, I. Rendina, P. Ferraro, and G. Coppola "Label-free biochemical characterization of bovine sperm cells using Raman microscopy", Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 87920E (23 May 2013); https://doi.org/10.1117/12.2021575

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KEYWORDS

Raman spectroscopy

Proteins

Signal to noise ratio

Spectroscopy

Microscopes

Biological research

Light scattering

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