Fluorescence lifetime imaging microscopy of nanodiamonds in vivo

Yung Kuo; Tsung-Yuan Hsu; Yi-Chun Wu; Jui-Hung Hsu; Huan-Cheng Chang

doi:10.1117/12.2004494

29 March 2013 Fluorescence lifetime imaging microscopy of nanodiamonds in vivo

Yung Kuo, Tsung-Yuan Hsu, Yi-Chun Wu, Jui-Hung Hsu, Huan-Cheng Chang

Proceedings Volume 8635, Advances in Photonics of Quantum Computing, Memory, and Communication VI; 863503 (2013) https://doi.org/10.1117/12.2004494
Event: SPIE OPTO, 2013, San Francisco, California, United States

Abstract

The negatively charged nitrogen-vacancy (NV^–) center in bulk diamond is a photostable fluorophore with a radiative lifetime of 11.6 ns at room temperature. The lifetime substantially increases to ~20 ns for diamond nanoparticles (size ~ 100 nm) suspended in water due to the change in refractive index of the surrounding medium of the NV^– centers. This fluorescence decay time is much longer than that (typically 1 − 4 ns) of endogenous and exogenous fluorophores commonly used in biological imaging, making it possible to detect NV^–-containing nanodiamonds in vivo at the single particle level by fluorescence lifetime imaging microscopy (FLIM). We demonstrate the feasibility of this approach using Caenorhabditis elegans (C. elegans) as a model organism.

Citation Download Citation

Yung Kuo, Tsung-Yuan Hsu, Yi-Chun Wu, Jui-Hung Hsu, and Huan-Cheng Chang "Fluorescence lifetime imaging microscopy of nanodiamonds in vivo", Proc. SPIE 8635, Advances in Photonics of Quantum Computing, Memory, and Communication VI, 863503 (29 March 2013); https://doi.org/10.1117/12.2004494

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