OCT guided microinjections for mouse embryonic research

Kirill V. Larin; Saba H. Syed; Andrew J. Coughlin; Shang Wang; Jennifer L. West; Mary E. Dickinson; Irina V. Larina

doi:10.1117/12.2006213

26 March 2013 OCT guided microinjections for mouse embryonic research

Kirill V. Larin, Saba H. Syed, Andrew J. Coughlin, Shang Wang, Jennifer L. West, Mary E. Dickinson, Irina V. Larina

Proceedings Volume 8593, Optical Methods in Developmental Biology; 85930E (2013) https://doi.org/10.1117/12.2006213
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Optical coherence tomography (OCT) is gaining popularity as live imaging tool for embryonic research in animal models. Recently we have demonstrated that OCT can be used for live imaging of cultured early mouse embryos (E7.5-E10) as well as later stage mouse embryos in utero (E12.5 to the end of gestation). Targeted delivery of signaling molecules, drugs, and cells is a powerful approach to study normal and abnormal development, and image guidance is highly important for such manipulations. Here we demonstrate that OCT can be used to guide microinjections of gold nanoshell suspensions in live mouse embryos. This approach can potentially be used for variety of applications such as guided injections of contrast agents, signaling molecules, pharmacological agents, cell transplantation and extraction, as well as other image-guided micromanipulations. Our studies also reveal novel potential for gold nanoshells in embryonic research.

Citation Download Citation

Kirill V. Larin, Saba H. Syed, Andrew J. Coughlin, Shang Wang, Jennifer L. West, Mary E. Dickinson, and Irina V. Larina "OCT guided microinjections for mouse embryonic research", Proc. SPIE 8593, Optical Methods in Developmental Biology, 85930E (26 March 2013); https://doi.org/10.1117/12.2006213

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