Differences in activity profile of bacterial cultures studied by dynamic speckle patterns

E. E. Ramírez-Miquet; I. Otero; D. Rodríguez; J. G. Darias; A. M. Combarro; O. R. Contreras

doi:10.1117/12.2000576

22 February 2013 Differences in activity profile of bacterial cultures studied by dynamic speckle patterns

E. E. Ramírez-Miquet, I. Otero, D. Rodríguez, J. G. Darias, A. M. Combarro, O. R. Contreras

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85871P (2013) https://doi.org/10.1117/12.2000576
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We outline the main differences in the activity profile of bacterial cultures studied by dynamic laser speckle (or biospeckle) patterns. The activity is detected in two sorts of culture mediums. The optical setup and the experimental procedure are presented. The experimentally obtained images are processed by the temporal difference method and a qualitative assessment is made with the time history of speckle patterns of the sample. The main differences are studied after changing the culture medium composition. We conclude that the EC medium is suitable to detect the E. coli bacterial presence in early hours and that Mueller Hinton agar delays some additional hours to make possible the assessment of bacteria in time.

Citation Download Citation

E. E. Ramírez-Miquet, I. Otero, D. Rodríguez, J. G. Darias, A. M. Combarro, and O. R. Contreras "Differences in activity profile of bacterial cultures studied by dynamic speckle patterns", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871P (22 February 2013); https://doi.org/10.1117/12.2000576

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