Raman fiberoptic probe for monitoring human tissue engineered oral mucosa constructs

Alexander Khmaladze; Shiuhyang Kuo; Paul Okagbare; Cynthia L. Marcelo; Stephen E. Feinberg; Michael D. Morris

doi:10.1117/12.2005546

15 February 2013 Raman fiberoptic probe for monitoring human tissue engineered oral mucosa constructs

Alexander Khmaladze, Shiuhyang Kuo, Paul Okagbare, Cynthia L. Marcelo, Stephen E. Feinberg, Michael D. Morris

Proceedings Volume 8579, Optical Interactions with Tissue and Cells XXIV; 85790L (2013) https://doi.org/10.1117/12.2005546
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

In oral and maxillofacial surgery, there is a need for tissue engineered constructs for dental implants, reconstructions due to trauma, oral cancer or congenital defects. A non-invasive quality monitoring of the fabrication of tissue engineered constructs during their production and implantation is a required component of any successful tissue engineering technique. We demonstrate the design and application of a Raman spectroscopic probe for rapid and noninvasive monitoring of Ex Vivo Produced Oral Mucosa Equivalent constructs (EVPOMEs). We conducted in vivo studies to identify Raman spectroscopic failure indicators for EVPOMEs (already developed in vitro), and found that Raman spectra of EVPOMEs exposed to thermal stress showed correlation of the band height ratio of CH₂ deformation to phenylalanine ring breathing modes, providing a Raman metric to distinguish between viable and nonviable constructs. This is the first step towards the ultimate goal to design a stand-alone system, which will be usable in a clinical setting, as the data processing and analysis will be performed with minimal user intervention, based on already established and tested Raman spectroscopic indicators for EVPOMEs.

Citation Download Citation

Alexander Khmaladze, Shiuhyang Kuo, Paul Okagbare, Cynthia L. Marcelo, Stephen E. Feinberg, and Michael D. Morris "Raman fiberoptic probe for monitoring human tissue engineered oral mucosa constructs", Proc. SPIE 8579, Optical Interactions with Tissue and Cells XXIV, 85790L (15 February 2013); https://doi.org/10.1117/12.2005546

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available