In vivo three-dimensional optical coherence tomography and multiphoton microscopy in a mouse model of ovarian neoplasia

Jennifer M. Watson; Samuel L. Marion; Photini Faith Rice; David L. Bentley; David Besselsen; Urs Utzinger; Patricia B. Hoyer; Jennifer K. Barton

doi:10.1117/12.1000273

19 March 2013 In vivo three-dimensional optical coherence tomography and multiphoton microscopy in a mouse model of ovarian neoplasia

Jennifer M. Watson, Samuel L. Marion, Photini Faith Rice, David L. Bentley, David Besselsen, Urs Utzinger, Patricia B. Hoyer, Jennifer K. Barton

Author Affiliations +

Proceedings Volume 8577, Optical Biopsy XI; 85770P (2013) https://doi.org/10.1117/12.1000273
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Our goal is to use optical coherence tomography (OCT) and multiphoton microscopy (MPM) to detect early tumor development in a mouse model of ovarian neoplasia. We hope to use information regarding early tumor development to create a diagnostic test for high-risk patients. In this study we collect in vivo images using OCT, second harmonic generation and two-photon excited fluorescence from non-vinylcyclohexene diepoxide (VCD)-dosed and VCD-dosed mice. VCD causes follicular apoptosis (simulating menopause) and leads to tumor development. Using OCT and MPM we visualized the ovarian microstructure and were able to see differences between non-VCD-dosed and VCD-dosed animals. This leads us to believe that OCT and MPM may be useful for detecting changes due to early tumor development.

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Jennifer M. Watson, Samuel L. Marion, Photini Faith Rice, David L. Bentley, David Besselsen, Urs Utzinger, Patricia B. Hoyer, and Jennifer K. Barton "In vivo three-dimensional optical coherence tomography and multiphoton microscopy in a mouse model of ovarian neoplasia", Proc. SPIE 8577, Optical Biopsy XI, 85770P (19 March 2013); https://doi.org/10.1117/12.1000273

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