17 October 2012Combined micro computed tomography and histology study of bone augmentation and distraction osteogenesis
Bernd Ilgenstein, Hans Deyhle, Claude Jaquiery, Christoph Kunz, Anja Stalder, Stefan Stübinger, Gernot Jundt, Felix Beckmann, Bert Müller, Simone Elke Hieber
Bernd Ilgenstein,1 Hans Deyhle,1 Claude Jaquiery,1 Christoph Kunz,1 Anja Stalder,1 Stefan Stübinger,2 Gernot Jundt,1 Felix Beckmann,3 Bert Müllerhttps://orcid.org/0000-0003-4078-9109,1 Simone Elke Hieber1
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Bone augmentation is a vital part of surgical interventions of the oral and maxillofacial area including dental
implantology. Prior to implant placement, sufficient bone volume is needed to reduce the risk of peri-implantitis. While
augmentation using harvested autologous bone is still considered as gold standard, many surgeons prefer bone substitutes
to reduce operation time and to avoid donor site morbidity. To assess the osteogenic efficacy of commercially available
augmentation materials we analyzed drill cores extracted before implant insertion. In younger patients, distraction
osteogenesis is successfully applied to correct craniofacial deformities through targeted bone formation. To study the
influence of mesenchymal stem cells on bone regeneration during distraction osteogenesis, human mesenchymal stem
cells were injected into the distraction gap of nude rat mandibles immediately after osteotomy. The distraction was
performed over eleven days to reach a distraction gap of 6 mm. Both the rat mandibles and the drill cores were scanned
using synchrotron radiation-based micro computed tomography. The three-dimensional data were manually registered
and compared with corresponding two-dimensional histological sections to assess bone regeneration and its morphology.
The analysis of the rat mandibles indicates that bone formation is enhanced by mesenchymal stem cells injected before
distraction. The bone substitutes yielded a wide range of bone volume and degree of resorption. The volume fraction of
the newly formed bone was determined to 34.4% in the computed tomography dataset for the augmentation material
Geistlich Bio-Oss®. The combination of computed tomography and histology allowed a complementary assessment for
both bone augmentation and distraction osteogenesis.
Bernd Ilgenstein,Hans Deyhle,Claude Jaquiery,Christoph Kunz,Anja Stalder,Stefan Stübinger,Gernot Jundt,Felix Beckmann,Bert Müller, andSimone Elke Hieber
"Combined micro computed tomography and histology study of bone augmentation and distraction osteogenesis", Proc. SPIE 8506, Developments in X-Ray Tomography VIII, 85060M (17 October 2012); https://doi.org/10.1117/12.929616
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Bernd Ilgenstein, Hans Deyhle, Claude Jaquiery, Christoph Kunz, Anja Stalder, Stefan Stübinger, Gernot Jundt, Felix Beckmann, Bert Müller, Simone Elke Hieber, "Combined micro computed tomography and histology study of bone augmentation and distraction osteogenesis," Proc. SPIE 8506, Developments in X-Ray Tomography VIII, 85060M (17 October 2012); https://doi.org/10.1117/12.929616