Closed loop adaptive optics with a laser guide star for biological light microscopy

Christopher D. Saunter; Cyril Bourgenot; John M. Girkin; Gordon D. Love

doi:10.1117/12.909927

15 February 2012 Closed loop adaptive optics with a laser guide star for biological light microscopy

Christopher D. Saunter, Cyril Bourgenot, John M. Girkin, Gordon D. Love

Proceedings Volume 8253, MEMS Adaptive Optics VI; 82530J (2012) https://doi.org/10.1117/12.909927
Event: SPIE MOEMS-MEMS, 2012, San Francisco, California, United States

Abstract

We report on the development of a widefield microscope that achieves adaptive optics correction through the use of a wavefront sensor observing an artificial laser guide star induced within the sample. By generating this guide star at arbitrary positions and depths within the sample we allow the delivery of high-resolution images. This approach delivers much faster AO correction than image optimization techniques, and allows the use of AO with fluorescent imaging modalities without generating excessive photo-toxic damage in the sample, or inducing significant photo-bleaching in the flurophore molecules.

Citation Download Citation

Christopher D. Saunter, Cyril Bourgenot, John M. Girkin, and Gordon D. Love "Closed loop adaptive optics with a laser guide star for biological light microscopy", Proc. SPIE 8253, MEMS Adaptive Optics VI, 82530J (15 February 2012); https://doi.org/10.1117/12.909927

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